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Forest plots showing the effect of 5–20 μΜ SFN on apoptosis indices in LM8 cells. ( a ) Distribution of LM8 cells in sub-G1 phase and active caspase-3 levels in LM8 cells after 24 h of treatment. Data from the included studies were categorized into subgroups based on the apoptosis indicator. ( b ) LM8 cell viability after 48 h treatment, as shown by the <t>trypan</t> <t>blue</t> <t>dye</t> <t>exclusion</t> <t>test.</t> Data from the included studies were categorized into subgroups based on the concentration of the SFN treatment. The green squares represent the estimated std. mean difference for each individual study and the black diamond represents the pooled std. mean difference and the associated 95% CI. The studies included in these forest plots are: Matsui et al. (2007) , Sawai et al. (2013) .
Trypan Blue Dye Exclusion Test, supplied by Sawai Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Forest plots showing the effect of 5–20 μΜ SFN on apoptosis indices in LM8 cells. ( a ) Distribution of LM8 cells in sub-G1 phase and active caspase-3 levels in LM8 cells after 24 h of treatment. Data from the included studies were categorized into subgroups based on the apoptosis indicator. ( b ) LM8 cell viability after 48 h treatment, as shown by the <t>trypan</t> <t>blue</t> <t>dye</t> <t>exclusion</t> <t>test.</t> Data from the included studies were categorized into subgroups based on the concentration of the SFN treatment. The green squares represent the estimated std. mean difference for each individual study and the black diamond represents the pooled std. mean difference and the associated 95% CI. The studies included in these forest plots are: Matsui et al. (2007) , Sawai et al. (2013) .
Trypan Blue Dye Exclusion Test, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Forest plots showing the effect of 5–20 μΜ SFN on apoptosis indices in LM8 cells. ( a ) Distribution of LM8 cells in sub-G1 phase and active caspase-3 levels in LM8 cells after 24 h of treatment. Data from the included studies were categorized into subgroups based on the apoptosis indicator. ( b ) LM8 cell viability after 48 h treatment, as shown by the <t>trypan</t> <t>blue</t> <t>dye</t> <t>exclusion</t> <t>test.</t> Data from the included studies were categorized into subgroups based on the concentration of the SFN treatment. The green squares represent the estimated std. mean difference for each individual study and the black diamond represents the pooled std. mean difference and the associated 95% CI. The studies included in these forest plots are: Matsui et al. (2007) , Sawai et al. (2013) .
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trypan blue exclusion test  (Bio-Rad)
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Forest plots showing the effect of 5–20 μΜ SFN on apoptosis indices in LM8 cells. ( a ) Distribution of LM8 cells in sub-G1 phase and active caspase-3 levels in LM8 cells after 24 h of treatment. Data from the included studies were categorized into subgroups based on the apoptosis indicator. ( b ) LM8 cell viability after 48 h treatment, as shown by the <t>trypan</t> <t>blue</t> <t>dye</t> <t>exclusion</t> <t>test.</t> Data from the included studies were categorized into subgroups based on the concentration of the SFN treatment. The green squares represent the estimated std. mean difference for each individual study and the black diamond represents the pooled std. mean difference and the associated 95% CI. The studies included in these forest plots are: Matsui et al. (2007) , Sawai et al. (2013) .
Trypan Blue Exclusion Test, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Forest plots showing the effect of 5–20 μΜ SFN on apoptosis indices in LM8 cells. ( a ) Distribution of LM8 cells in sub-G1 phase and active caspase-3 levels in LM8 cells after 24 h of treatment. Data from the included studies were categorized into subgroups based on the apoptosis indicator. ( b ) LM8 cell viability after 48 h treatment, as shown by the trypan blue dye exclusion test. Data from the included studies were categorized into subgroups based on the concentration of the SFN treatment. The green squares represent the estimated std. mean difference for each individual study and the black diamond represents the pooled std. mean difference and the associated 95% CI. The studies included in these forest plots are: Matsui et al. (2007) , Sawai et al. (2013) .

Journal: Biomedicines

Article Title: Sulforaphane’s Role in Osteosarcoma Treatment: A Systematic Review and Meta-Analysis of Preclinical Studies

doi: 10.3390/biomedicines13051048

Figure Lengend Snippet: Forest plots showing the effect of 5–20 μΜ SFN on apoptosis indices in LM8 cells. ( a ) Distribution of LM8 cells in sub-G1 phase and active caspase-3 levels in LM8 cells after 24 h of treatment. Data from the included studies were categorized into subgroups based on the apoptosis indicator. ( b ) LM8 cell viability after 48 h treatment, as shown by the trypan blue dye exclusion test. Data from the included studies were categorized into subgroups based on the concentration of the SFN treatment. The green squares represent the estimated std. mean difference for each individual study and the black diamond represents the pooled std. mean difference and the associated 95% CI. The studies included in these forest plots are: Matsui et al. (2007) , Sawai et al. (2013) .

Article Snippet: Trypan blue dye exclusion test , Matsui et al., 2007 [ ]; Sawai et al., 2013 [ ] , LM8 (murine) , 5 μΜ 10 μΜ 20 μΜ , 24 , −3.16 (−5.88, −0.45) −5.21 (−9.42, −0.99) −9.49 (−16.61, −2.37) , −4.30 (−6.47, −2.12) , Std. mean difference, IV, Fixed effects, 95% CI , Chi 2 = 7.49, df = 5, p = 0.19, I 2 = 33% , Z = 3.87, p = 0.0001.

Techniques: Concentration Assay